Cyanide removal from waste water

Simultaneous treatment of phenol and cyanide containing synthetic/simulated wastewater using mixed culture immobilized on coconut shell activated carbon biomass in a packed bed bio-column reactor

Neetu Singh, Chandrajit Balomajumder


The removal of phenol and cyanide from synthetic/simulated waste water using a packed bed bio-column reactor was investigated. Mixed culture of Pseudomonas putida MTCC 1194 and Serretia odoriferra MTCC 5700 has been immobilized on the iron impregnated coconut shell activated carbon (Fe-CSAC)  bed in the packed bed bio-column reactor. The synthetic/simulated waste water sample comprising phenol and cyanide at initial concentration 500 and 50 mg/L, respectively, was used. Concentrations of phenol and cyanide have been found to be decreased, lower than permissible limits in the treated water. The significant influence of bed height (cm) and empty bed contact time (EBCT) was examined onto the simultaneous removal of phenol and cyanide in the packed bed bio-column reactor. On the other hand, after 4-5 days of continuous treatment of phenol and cyanide the influence of process parameters was not noteworthy. Dissolved oxygen (D.O.) has been found to decline along with time and the pH of the solution in the packed bed bio-column reactor has decreased initially for 2 days but after that it became the same as the influent. Real industrial wastewater samples can be efficiently handled.


Bio-Column Reactor; Cyanide; Mixed Culture; Phenol; P.putida; S.odorifera;

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