Antiquorum sensing activity of selected Eritrean traditional medicinal plants against Pseudomonas aeruginosa
Abstract
The discovery of bacterial-communication systems (quorum-sensing systems), which orchestrate important temporal events during the infection process, has afforded a novel opportunity to ameliorate bacterial infection by means other than growth inhibition. In this study three different assays were performed; Las-A staphylolytic assay, anti-swarming assay, pyocyanin assay. In the anti-swarming activity, the highest anti-swarming activity were seen in the chloroform and petroleum extract of O.fruticosa. In the Las-A activity, the highest reduction in Las-A activity was seen in the chloroform extract of V.sinaiticum (70.5%) and also petroleum extract of V.sinaticum and chloroform extract O.fructicosa (70.3%).In the Pyocyanin assay, the chloroform extract of O.fructicosa and V.sinaiticum showed more significant effect in decreasing the Pyocyanin production by 81.8% and 79.5% respectively.
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A.R. Singh. Modern medicine: Towards prevention, cure, well-being and longevity, Mens Sana Monographs. 2010, 8(1), 17–29.
A. Smith. Bacterial resistance to antibiotics. Hugo and Russell’s Pharmaceutical Microbiology. 2004 Aug 2:220-3.
P.S. Stewart, J.W. Costerton. Antibiotic resistance of bacteria in biofilms. The lancet. 2001, 358(9276), 135-8.
X.Z. Li, P. Plésiat, H. Nikaido. The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria. Clinical microbiology reviews. 2015, 28(2), 337-418.
N. Høiby, T. Bjarnsholt, M. Givskov, S. Molin, O. Ciofu. Antibiotic resistance of bacterial biofilms. International journal of antimicrobial agents. 2010, 35(4), 322-32.
A. Smith. Bacterial resistance to antibiotics. Hugo and Russell’s Pharmaceutical Microbiology. 2004 Aug 2, 220-3.
C.L. Koh, C.K. Sam, W.F. Yin, L.Y. Tan, T. Krishnan, Y.M. Chong, K.G. Chan. Plant-derived natural products as sources of anti-quorum sensing compounds. Sensors. 2013, 13(5), 6217-28.
P. Jiménez-Gómez, M.J. Pozuelo de Felipe, F. Llinares Pinell, J.E. Garcia de los Rios. Quorum-sensing in Pseudomonas aeruginosa and Salmonella: Active natural compounds as antagonists. Commun Curr Res Edu Topics Trends App Microbiol. 2007, 1, 41-51.
A.L. Adonizio, K. Downum, B.C. Bennett, K. Mathee. Anti-quorum sensing activity of medicinal plants in southern Florida. Journal of ethnopharmacology. 2006, 105(3), 427-35.
A. Adonizio, K.F. Kong, K. Mathee. Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrobial agents and chemotherapy. 2008, 52(1), 198-203.
K.A. McDonough, A. Rodriguez. The myriad roles of cyclic AMP in microbial pathogens: from signal to sword. Nature reviews Microbiology. 2012, 10(1), 27-38.
B. Sultana, F. Anwar, M. Ashraf. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules. 2009, 14(6), 2167-80.
H. Kazemian, S. Ghafourian, H. Heidari, P. Amiri, J.K. Yamchi, A. Shavalipour, H. Houri, A, Maleki, N. Sadeghifard. Antibacterial, anti-swarming and anti-biofilm formation activities of Chamaemelum nobile against Pseudomonas aeruginosa. Revista da Sociedade Brasileira de Medicina Tropical. 2015, 48(4), 432-6.
J.D. Shrout, D.L. Chopp, C.L. Just, M. Hentzer, M. Givskov, M.R. Parsek. The impact of quorum sensing and swarming motility on Pseudomonas aeruginosa biofilm formation is nutritionally conditional. Molecular microbiology. 2006, 62(5), 1264-77.
K.S. Musthafa, A.V. Ravi, A. Annapoorani, I.S. Packiavathy, S.K. Pandian. Evaluation of anti-quorum-sensing activity of edible plants and fruits through inhibition of the N-acyl-homoserine lactone system in Chromobacterium violaceum and Pseudomonas aeruginosa. Chemotherapy. 2010, 56(4), 333-9.
W. Fabry, P.O. Okemo, R. Ansorg. Antibacterial activity of East African medicinal plants. Journal of ethnopharmacology. 1998, 60(1), 79-84.
H. Jafri, F. Husain, I. Ahmad. Antibacterial and antibiofilm activity of some essential oils and compounds against clinical strains of Staphylococcus aureus. J. Biomed. Therapeutic Sci., 2014, 1(1), 65-71.
M. Piplani, A., Rana, P. Sharma. Synthesis, characterization and evaluation of prodrugs of ciprofloxacin clubbed with benzothiazoles through N-Mannich base approach. Chem. Biol. Lett., 2016, 3(2), 52-57.
M.S. Khan, M. Zahin, S. Hasan, F.M. Husain, I. Ahmad I. Inhibition of quorum sensing regulated bacterial functions by plant essential oils with special reference to clove oil. Lett. Applied Microbiol., 2009, 49(3), 354-60.
I. Singh. Antimicrobials in Higher Plants: classification, mode of action and bioactivities. Chem. Biol. Lett., 2017, 4(1), 48-62.
L.H. Zhang, Y.H. Dong. Quorum sensing and signal interference: diverse implications. Mol. Microbiol., 2004, 53(6), 1563-71.
J. Singh, S. Kumar, B. Rathi, K. Bhrara, B.S. Chhikara. Therapeutic analysis of Terminalia arjuna plant extracts in combinations with different metal nanoparticles. J. Mat. NanoScience, 2015, 2(1), 1-7.
N. Gupta, C. Gupta, S. Sharma, R. Sharma, H. Bohidar. Comparative study of antibacterial activity of standard antibiotic with silver nanoparticles synthesized using ocimum tenuiflorum and garcinia mangostana leaves. Chem. Biol. Lett., 2015, 2(2), 41-44.
T. Hertiani, S.U. Pratiwi. Hydnophytum formicarum Jack ethanol extract modulates quorum sensing-controlled pathogenicity in Pseudomonas aeruginosa. Pak. J. Pharm. Sci. 2015, 28(5), 1691-7.
A. Tamrakar, A. Singh, M. Chodhrary, P. Kodgire. Fighting with Gram-negative enemy: Can outer membrane proteins aid in the rescue?. Chem. Biol. Lett., 2017, 4(1), 9-19.
S.S. Yeo, F.Y. Tham. Anti-quorum sensing and antimicrobial activities of some traditional Chinese medicinal plants commonly used in South-East Asia. Malaysian Journal of Microbiology. 2012, 8(1), 11-20.
A.L. Adonizio. Anti-quorum sensing agents from South Florida medicinal plants and their attenuation of Pseudomonas aeruginosa pathogenicity (Doctoral dissertation, Florida International University).
S. Mishra, M. Khatri, V. Mehra. Trials and tribulations in tuberculosis research: Can plant based drug(s) be the solution?. Chem. Biol. Lett., 2017, 4(1), 33-47.
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