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Screenings of antimicrobial, cytotoxic and anti-inflammatory potentials of crude methanolic extracts of Crinum latifolium (Leaves)

Md. Saddam Hussain, Md. Abdur Rahman, Md. Shalahuddin Millat, Mithun Chandra Roy, Mohammad Tohidul Amin, Md. Mizanur Rahman Moghal


Crude methanolic extract of Crinum latifolium plant (Leaves) was assayed to identify various pharmacological properties. Antimicrobial potential of crude methanolic extracts of Crinum latifolium was accomplished by most commonly used disc diffusion method against a wide range of Gram positive and Gram negative bacteria. Extracts showed slight antimicrobial activity against Gram positive bacteria while surprisingly showed significant antimicrobial activity against Gram negative bacteria E. coli. In contrast to Vincristine Sulphate, the crude metanolic, n-hexane soluble, petroleum ether soluble and chloroform soluble extracts showed slight to moderate cytotoxic properties with LC50 value of 7.06µg/ml, 48.978µg/ml, 242.83µg/ml and 153.93µg/ml respectively. Plant extract showed significant anti-inflammatory properties i.e 16.21% & 20.55%10mg/ml for hypotonic solution and heat induced condition respectively. So, this plant extract demands further research for revealing all its potency to have new safe drug for the entire respective field of medical science.


Crinum latifolium; Zone of inhibition; Brine shrimp lethality bioassay; anti-inflammatory;

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W.C. Evans. Treaseand Evanspharmacognosy. London: WB Saunders 2002.

J. Ueda, Y. Tezuka, A.H. Banskota, Q. Tran, Harimayay, SaikiI, etal. Antiproliferative activity of Vietnamese medicinal plants. Biol Pharm Bull. 2002, 25, 753-60.

S.E. Kintzios, M.G. Barberaki. Plants that fight cancer. Boca Raton: CRCPress. 2004.

J. Bauer, R. Rojas, B. Bustamante. Antimicrobial activity of selected Peruvian medicinal plants. J Ethnopharmacol. 2003, 88, 199- 204.

A. Tamrakar, A. Singh, M. Chodhrary, P. Kodgire. Fighting with Gram-negative enemy: Can outer membrane proteins aid in the rescue?. Chemical Biology Letters, 2017, 4(1), 9-19.

D. Runyoro, M. Matee, N. Olipa, C. Joseph, H. Mbwambo. Screening of Tanzanian medicinal plants for anti-Candida activity. BMC Complement Altern Med. 2006, 6, 11.

V.Rajendran, K.S.Lakshmi. In vitro and In vivo anti-inflammatory activity of leaves of Symplocos cochinchnensis (Lour) Mooressp laurina.Bangladesh. J. Pharmacol.2008, 3, 121.

J.Omale, P.N.Okafor. Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata. Afr. J. Biotechnol. 2008, 7, 3129-3133.

R.A.Naibi, V.S. Sethuraman, N.Suluchana, J.Sadique. Satellite symposium on traditional medicine as Asian congress of Pharmacology.Tamil University of Thanjavur.1985, 140.

O.O.Oyedapo, F.C.Sab, J.A.Olagunju. Bioactivity of fresh leaves of Lantana camara. Biomed. Lett. 1999, 59, 175- 183.

S.M.R. Dewan, D. Abhijit. Investigation of in vitro thrombolytic potential and phytochemical nature of crinum latifolium leaves growing in coastal region of Bangladesh. International Journal of Biological & Pharmaceutical Research. 2013, 4(1), 1-7.

N. Gupta, C. Gupta, S. Sharma, R.K. Sharma, H.B. Bohidar. Comparative study of antibacterial activity of standard antibiotic with silver nanoparticles synthesized using ocimum tenuiflorum and garcinia mangostana leaves. Chemical Biology Letters, 2015, 2(2), 41-44.

A. Mittal, K. Thanki, S. Jain, U. Banerjee. Comparative studies of anticancer and antimicrobial potential of bioinspired silver and silver-selenium nanoparticles. Appl. Nanomed., 2016, 1(1), 1-6.

W.C. Evans. Trease and Evans Pharmacognosy (Microbial Assay). WB Saunders Company Ltd, London.1996,14, 473.

A. Bauer, W. Kirby, J.C. Sherris, M. Turck. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 1966, 45, 493.

S. Prabhu, S.J. Britto, P. Thangavel, L. Raj. Antibacterial and Antioxidant Activity of Leaves of Canavalia Mollis Wight and Arn (Horse Bean). Int. J. Pharm. Sci. Drug Res 2011, 2.

H. Jafri, F. Husain, I. Ahmad. Antibacterial and antibiofilm activity of some essential oils and compounds against clinical strains of Staphylococcus aureus. J. Biomedical Therapeutic Sciences, 2014, 1(1), 65-71.

A.Opler, R.Mizell, A.Robert, M.Cervantes-Cervantes, D.Kincaid, E.J.Kennelly. Use of a brine shrimp assay to study herbal teas in the classroom. The American Biology Teacher. 2002, 64, 8596-8604.

B.N. Meyer, N.R. Ferrigni, J.E. Putnam, L.B. Jacobsen, D.E. Nichols, J.L. McLaughlin .Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982, 45, 31–34.

A.V. Krishnaraju, T.V.N. Rao, D. Sundararaju, M. Vanisree, H.S. Tsay, G.V. Subbaraju. Biological Screening of Medicinal Plants Collected from Eastern Ghats of India Using Artemia salina (Brine Shrimp Test). International Journal of Applied Science and Engineering. 2006, 4(2), 115-125.

M.K.Islam, Z.EtiI,J.A.Chowdury.Cytotoxic Studies on Two Meliaceae plants: Chukrasia Tabularis and Aglaia Roxburghiana. J. Sci. Res. 2009, 1(2), 399-403

A.Scalbert. Antimicrobial properties of tannin. Phytochemistry 1991, 30, 3875-3883.

K.T. Chung, T.Y. Wong, Y.Wei, Y.W. Huang, Y. Lin. Tannins and human health. A review. Crit Rev Food Sci Nutr. 1998, 8, 421-464.

T.A. Geissman. Flavonoid compounds, tannins, lignins and related compounds. In: Florkin M and Stotz EH (eds). Pyrrole Pigments, Isoprenoid Compounds and Phenolic Plant Constituents, New York, USA: Elsevier Press. 1963, 265.

M.M. Cowan. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999, 12, 564-582.

P. Kumari, R. Gautam, A. Milhotra. Application of Porphyrin nanomaterials in Photodynamic therapy. Chem Biol Lett, 2016, 3(2), 32-37.

Half E, Arber N. Colon cancer: Preventive agents and the present status of chemoprevention. Exp. Opin. Pharmacother. 2009, 10, 211–219.

D.S. Michaud, D. Feskanich, E.B. Rimm. Intake of specific carotenoids and risk of lung cancer in two prospective U.S. cohorts. Am J Clin Nutr. 2000, 72, 990-997.

E.R. Greenberg, J.A. Baron, T.D. Tosteson. A clinical trial of antioxidant vitamins to prevent colorectal cancer. N Engl J Med. 1994, 331, 141-147.

D.F. Birt, S. Hendrich, W.Q. Wang. Dietary agents in cancer prevention: flavanoids and isoflavonoids. Pharmacol Ther. 2001, 90, 157–177.

J. Singh, S. Kumar, B. Rathi, K. Bhrara, B.S. Chhikara. Therapeutic analysis of Terminalia arjuna plant extracts in combinations with different metal nanoparticles. J. Mat. NanoSci,, 2015, 2(1), 1-7.

J.E. Middleton. Biological properties of plant flavonoids: An overview. Int. J. Pharmacognosy. 1996, 34, 344-348.


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